Application
Immunohistochemistry of RNF20 in human liver tissue with RNF20 antibody at 2.5 μg/ml.Application
Western blot analysis of RNF20 in human liver tissue lysate with RNF20 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of RNF20 in human liver tissue with RNF20 antibody at 20 μg/ml.| Product Name | RNF20 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNF20 Antibody: BRE1, BRE1A, hBRE1, E3 ubiquitin-protein ligase BRE1A, RING finger protein 20, BRE1-A |
| Product description | RNF20 Antibody: RNF20 is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. RNF20 selectively suppresses the expression of some genes such as several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS). In contrast, RNF20 also positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. Together with the deubiquitinase USP44, RNF20 regulates stem cell differentiation by modulating H2B monoubiquitination.1) Zhu B, Zheng Y, Pham AD, et al. Monoubiquitination of human histone H2B: the factors involved and their roles in HOX gene regulation. Mol. Cell 2005; 20:601-11. |
| Immunogen | RNF20 antibody was raised against a 19 amino acid peptide near the amino terminus of human RNF20. The immunogen is located within amino acids 130 - 180 of RNF20. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| RNF20 antibody can be used for detection of RNF20 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RNF20 |
|---|---|
| Alternative Names | RNF20 Antibody: BRE1 BRE1A hBRE1 E3 ubiquitin-protein ligase BRE1A RING finger protein 20 BRE1-A |
| Molecular Weight(MW) | Predicted: 99, 107 kDa Observed: 100, 110 kDa |
| Sequence Similarities | 2) Cat. No. 10-201 - Human Liver Tissue Slide |
| Tissue Specificity | Multiple isoforms of RNF20 are known to exist. |
Application
Immunohistochemistry of RNF20 in human liver tissue with RNF20 antibody at 2.5 μg/ml.
Application
Western blot analysis of RNF20 in human liver tissue lysate with RNF20 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of RNF20 in human liver tissue with RNF20 antibody at 20 μg/ml.| Positive Control | 1) Cat. No. 1304 - Human Liver Tissue Lysate |
|---|---|
| Application Notes | RNF20 antibody can be used for detection of RNF20 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RNF20 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | RNF20 Antibody is supplied in PBS containing 0.02% sodium azide. |
