Application
Immunohistochemistry of ROCK2 in mouse brain tissue with ROCK2 antibody at 5 μg/mL.Application
Western blot analysis of ROCK2 in mouse brain tissue lysate with ROCK2 antibody at (A) 1 and (B) 2 μg/mLApplication
Immunofluorescence of ROCK2 in mouse brain tissue with ROCK2 antibody at 20 μg/mL.| Product Name | ROCK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ROCK2 Antibody: ROCK2 is a member of the AGC serine/threonine protein kinase family that regulates the assembly of the actin cytoskeleton. ROCK2 contains a protein kinase domain, a coiled-coil region and a zinc finger region and appears to be present as a dimer. ROCK2, like its isozyme ROCK1, is a downstream target of the small G-protein Rho and plays a role in smooth muscle contraction, malignant cell transformation, tumor invasion and metastasis, and mediates the cellular morphological changes during apoptosis.1) Leung T, Chen XQ, Manser E, et al. The p160 RhoA-binding kinase ROK alpha is a member of a kinase family and is involved in the reorganization of the cytoskeleton. Mol. Cell Biol. 1996; 16:5313-27. |
| Immunogen | ROCK2 antibody was raised against a 15 amino acid synthetic peptide near the center of human ROCK2. The immunogen is located within amino acids 920 - 970 of ROCK2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ROCK2 antibody can be used for detection of ROCK2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ROCK2 |
|---|---|
| Alternative Names | ROCK2 Antibody: ROCK-II KIAA0619 Rho-associated protein kinase 2 Rho kinase 2 ROCK-II |
| Molecular Weight(MW) | Predicted: 153 kDa Observed: 160 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (100%) |
| Tissue Specificity | Three isoforms of ROCK2 are known to exist; this antibody recognizes all three isoforms. ROCK2 antibody is predicted to not cross-react with other ROCK protein family members. |
Application
Immunohistochemistry of ROCK2 in mouse brain tissue with ROCK2 antibody at 5 μg/mL.
Application
Western blot analysis of ROCK2 in mouse brain tissue lysate with ROCK2 antibody at (A) 1 and (B) 2 μg/mL
Application
Immunofluorescence of ROCK2 in mouse brain tissue with ROCK2 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | ROCK2 antibody can be used for detection of ROCK2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | ROCK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ROCK2 Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM290221