Application
Antibody used in WB on Human HepG2 at 0.3125 ug/ml.Application
Antibody used in IHC on Human Lung at 4.0-8.0 ug/ml.| Product Name | RPL8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RPL8, L8 |
| Product description | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL8 is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm.Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.1) Kenmochi, N., (1998) Genome Res. 8 (5), 509-523. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RPL8. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| RPL8 antibody can be used for detection of RPL8 by ELISA at 1:1562500. RPL8 antibody can be used for detection of RPL8 by western blot at 0.3125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
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| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RPL8 |
|---|---|
| Alternative Names | RPL8 L8 |
| Molecular Weight(MW) | 28 kDa, 28 kDa |
Application
Antibody used in WB on Human HepG2 at 0.3125 ug/ml.
Application
Antibody used in IHC on Human Lung at 4.0-8.0 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | RPL8 antibody can be used for detection of RPL8 by ELISA at 1:1562500. RPL8 antibody can be used for detection of RPL8 by western blot at 0.3125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store RPL8 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
