Application
Antibody used in WB on Human Jurkat 1 ug/ml.Application
Antibody used in WB on Human 721_B cells at 1 ug/ml.Application
Antibody used in WB on Human MCF7 at 1 ug/ml.Application
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.Application
Antibody used in WB on Hum. Fetal Brain at 1 ug/ml.Application
Antibody used in WB on Human Hela at 1 ug/ml.Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.Application
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.| Product Name | RPS14 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RPS14, S14, EMTB |
| Product description | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS14 is a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor.Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.1) Kenmochi, N., (1998) Genome Res. 8 (5), 509-523. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RPS14. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| RPS14 antibody can be used for detection of RPS14 by ELISA at 1:12500. RPS14 antibody can be used for detection of RPS14 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogDrosophilaHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RPS14 |
|---|---|
| Alternative Names | RPS14 S14 EMTB |
| Molecular Weight(MW) | 17 kDa, 16 kDa, 16 kDa |
| Entrez Gene | 6208 |
|---|---|
| Protein Accession | NP_001020242 |
Application
Antibody used in WB on Human Jurkat 1 ug/ml.
Application
Antibody used in WB on Human 721_B cells at 1 ug/ml.
Application
Antibody used in WB on Human MCF7 at 1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Brain at 1 ug/ml.
Application
Antibody used in WB on Human Hela at 1 ug/ml.
Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.
Application
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | RPS14 antibody can be used for detection of RPS14 by ELISA at 1:12500. RPS14 antibody can be used for detection of RPS14 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store RPS14 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
