Application
Immunofluorescence of RP S6 Kinase in Jurkat cells with RP S6 Kinase antibody at 20 μg/mL.Application
Immunocytochemistry of RPS6K1 in Jurkat cells with RPS6K1 antibody at 10 μg/mL.Application
Western blot analysis of RPS6K1 in Jurkat cell lysate with RPS6K1 antibody at (A) 2.5, (B) 5 and (C) 10 μg/mL.| Product Name | RPS6K1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RPS6K1 Antibody: RSK, HU-1, RSK1, MAPKAPK1A, Ribosomal protein S6 kinase alpha-1, 90 kDa ribosomal protein S6 kinase 1, S6K-alpha-1 |
| Product description | RPS6K1 Antibody: Ribosomal protein S6 kinase 1 (RPS6K1) is the best characterized effector of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. Nutrients and growth factors stimulate a complex including TOR, raptor (regulatory associated protein of TOR), and GbetaL to phosphorylate RPS6K1 and the eukaryotic initiation factor 4E binding protein (4EBP1), leading to increased protein synthesis and cell growth. RPS6K1 is thought to desensitize tissues to insulin as mice deficient in RPS6K1 have been shown to be hypersensitive to insulin and impervious to obesity-induced insulin resistance seen in wild type obese mice.1) Burnett PE, Barrow RK, Cohen NA, et al. RAFT1 phosphorylation of the translational regulators p70 S6 kinase and 4E-BP1. Proc. Natl. Acad. Sci. USA. 1998; 95:1432-7. |
| Immunogen | RPS6K1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human RPS6K1. The immunogen is located within amino acids 600 - 650 of RPS6K1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| RPS6K1antibody can be used for the detection of RPS6K1 by Western blot at 2.5 - 10 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPS6KA1 |
|---|---|
| Alternative Names | RPS6K1 Antibody: RSK HU-1 RSK1 MAPKAPK1A Ribosomal protein S6 kinase alpha-1 90 kDa ribosomal protein S6 kinase 1 S6K-alpha-1 |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
Application
Immunofluorescence of RP S6 Kinase in Jurkat cells with RP S6 Kinase antibody at 20 μg/mL.
Application
Immunocytochemistry of RPS6K1 in Jurkat cells with RPS6K1 antibody at 10 μg/mL.
Application
Western blot analysis of RPS6K1 in Jurkat cell lysate with RPS6K1 antibody at (A) 2.5, (B) 5 and (C) 10 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | RPS6K1antibody can be used for the detection of RPS6K1 by Western blot at 2.5 - 10 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RPS6K1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RPS6K1 Antibody is supplied in PBS containing 0.02% sodium azide. |
