Application
Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded mouse brain tissue reacted with RPS6KB2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of RPS6KB2 Antibody in K562 cell line lysates (35ug/lane)| Product Name | RPS6KB2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ribosomal protein S6 kinase beta-2, S6K-beta-2, S6K2, 70 kDa ribosomal protein S6 kinase 2, P70S6K2, p70-S6K 2, S6 kinase-related kinase, SRK, Serine/threonine-protein kinase 14B, p70 ribosomal S6 kinase beta, S6K-beta, p70 S6 kinase beta, p70 S6K-beta, p7 |
| Product description | RPS6KB2 is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates the S6 ribosomal protein and eucaryotic translation initiation factor 4B. Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation.1) Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). |
| Immunogen | This RPS6KB2 antibody is generated from rabbits immunized with human RPS6KB2 recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPS6KB2 |
|---|---|
| Alternative Names | Ribosomal protein S6 kinase beta-2 S6K-beta-2 S6K2 70 kDa ribosomal protein S6 kinase 2 P70S6K2 p70-S6K 2 S6 kinase-related kinase SRK Serine/threonine-protein kinase 14B p70 ribosomal S6 kinase beta S6K-beta p70 S6 kinase beta p70 S6K-beta p7 |
| Molecular Weight(MW) | 53 kDa |
Application
Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded mouse brain tissue reacted with RPS6KB2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of RPS6KB2 Antibody in K562 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
