RUNX3 belongs to The RUNX family of transcription factors that are important regulators of linage-specific gene expression in major developmental pathways. Runx3 is highly expressed in developing cranial and dorsal root ganglia (DRGs).1) Yarmus, M., et al., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (19), 7384-7389.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse RUNX3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
RUNX3 antibody can be used for detection of RUNX3 by ELISA at 1:12500. RUNX3 antibody can be used for detection of RUNX3 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Mouse NIH-3T3 at 1.25 ug/ml.
Positive Control
1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Application Notes
RUNX3 antibody can be used for detection of RUNX3 by ELISA at 1:12500. RUNX3 antibody can be used for detection of RUNX3 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store RUNX3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt