Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Antibody is used in Western blot to detect RYK in Jurkat cell lysate.Application
Western blot analysis of RYK using rabbit polyclonal RYK Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RYK gene (Lane 2).Application
Western blot analysis of lysate from human ovary tissue lysate, using RYK Antibody (N175) at 1:1000.Application
Western blot analysis of lysates from mouse lung and ovary tissue lysate(from left to right), using RYK Antibody (N175) at 1:1000 at each lane.Application
Western blot analysis of lysate from human ovary tissue lysate, using RYK Antibody (N175) at 1:1000.Application
Western blot analysis of lysate from mouse NIH/3T3 cell line, using RYK Antibody (N175) at 1:1000.| Product Name | RYK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tyrosine-protein kinase RYK, RYK, JTK5A |
| Product description | RYK is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. A nine nucleotide insertion in some transcripts results in the SLG variant. It is not established whether this is a product of alternative splicing or a second gene, since evidence for a second gene or pseudogene on chromosome 17 exists.1) Trivier, E., et al., J. Biol. Chem. 277(25):23037-23043 (2002). Katso, R.M., et al., Mol. Cell. Biol. 19(9):6427-6440 (1999). Wang, X.C., et al., Mol. Med. 2(2):189-203 (1996). Tamagnone, L., et al., Oncogene 8(7):2009-2014 (1993). Stacker, S.A., et al., Oncogene 8(5):1347-1356 (1993). |
| Immunogen | This RYK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-190 amino acids from human RYK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RYK |
|---|---|
| Alternative Names | Tyrosine-protein kinase RYK RYK JTK5A |
| Molecular Weight(MW) | 68 kDa |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Antibody is used in Western blot to detect RYK in Jurkat cell lysate.
Application
Western blot analysis of RYK using rabbit polyclonal RYK Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RYK gene (Lane 2).
Application
Western blot analysis of lysate from human ovary tissue lysate, using RYK Antibody (N175) at 1:1000.
Application
Western blot analysis of lysates from mouse lung and ovary tissue lysate(from left to right), using RYK Antibody (N175) at 1:1000 at each lane.
Application
Western blot analysis of lysate from human ovary tissue lysate, using RYK Antibody (N175) at 1:1000.
Application
Western blot analysis of lysate from mouse NIH/3T3 cell line, using RYK Antibody (N175) at 1:1000.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
