Application
Immunohistochemistry of S1PR2 in mouse brain tissue with S1PR2 antibody at 10 μg/ml.Application
Western blot analysis of S1PR2 in HeLa cell lysate with S1PR2 antibody at 1 μg/ml.Application
Immunofluorescence of S1PR2 in mouse brain tissue with S1PR2 antibody at 5 μg/ml.| Product Name | S1PR2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Sphingosine 1-phosphate receptor 2, S1P2, AGR16, EDG-5, EDG5, Gpcr13, H218, LPB2 |
| Product description | The Sphingosine 1-phosphate receptor 2 (S1PR2) protein was initially identified as G-protein coupled receptor thought to be involved in development (1) and was found to mediate sphingosine 1-phosphate (SPP)-induced cell rounding and neurite retraction (2). S1PR2 and the related protein S1PR3 (also known as EDG3) are also thought to mediate the SPP-induced cell proliferation, survival, and related signaling events (3). S1PR2 has also been found to be key regulator in acute vascular inflammation and may be a novel therapeutic target for vascular disorders (4).1) MacLennan AJ, Browe CS, Gaskin AA, et al. Cloning and characterization of a putative G-protein coupled receptor potentially involved in development. Mol. Cell Neurosci. 1994; 5:201-9. |
| Immunogen | S1PR2 antibody was raised against an 16 amino acid peptide near the carboxy terminus of human S1PR2. The immunogen is located within amino acids 270 - 320 of S1PR2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| WB:1:500~1:2000 IHC-P:1:50~1:200 IF:1:100~1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | S1PR2 |
|---|---|
| Alternative Names | Sphingosine 1-phosphate receptor 2 S1P2 AGR16 EDG-5 EDG5 Gpcr13 H218 LPB2 |
| Molecular Weight(MW) | Predicted: 39 kDa Observed: 50 kDa |
| Tissue Specificity | S1PR2 antibody is human, mouse and rat reactive. |
Application
Immunohistochemistry of S1PR2 in mouse brain tissue with S1PR2 antibody at 10 μg/ml.
Application
Western blot analysis of S1PR2 in HeLa cell lysate with S1PR2 antibody at 1 μg/ml.
Application
Immunofluorescence of S1PR2 in mouse brain tissue with S1PR2 antibody at 5 μg/ml.| Positive Control | HeLa Cell Lysate |
|---|---|
| Application Notes | WB:1:500~1:2000 IHC-P:1:50~1:200 IF:1:100~1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | S1PR2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | S1PR2 antibody is supplied in PBS containing 0.02% sodium azide. |
