Application
Immunofluorescence staining of methanol-fixed HeLa cells using S6 Ribosomal Protein (Phospho-Ser235).Application
Western blot analysis of lysed extracts from PC12 cells untreated or treated with anisomycin using S6 Ribosomal Protein (Phospho-Ser235).| Product Name | S6 Ribosomal Protein (phospho Ser235) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | S6, Mtapt, Tau, NP33, RPS6, RS6 |
| Product description | May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.1) McBride K, et al. (1998) Mol Cell Biol 18 (9): 5073-5081. Williams AJ, et al. (2003) Plant Physiol 132 (4): 2086-2097. Wilson MA, et al. (1997) Biochem J 325 (Pt 1): 217-222. Arnesen T, et al. (2005) Biochem J 386 (Pt 3): 433-443. |
| Immunogen | S6 Ribosomal Protein (Phospho-Ser235) antibody was raised against a peptide sequence around phosphorylation site of serine 235 (R-L-S (p) -S-L) derived from Human S6 Ribosomal Protein. |
| Modification | p-Ser235 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFWB |
| Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPS6 |
|---|---|
| Alternative Names | S6 Mtapt Tau NP33 RPS6 RS6 |
| Molecular Weight(MW) | 32 kDa |
| Tissue Specificity | This antibody detects endogenous level of S6 Ribosomal protein only when phosphorylated at serine 235. |
| Entrez Gene | 6194 |
|---|---|
| Protein Accession | NP_001001.2 |
Application
Immunofluorescence staining of methanol-fixed HeLa cells using S6 Ribosomal Protein (Phospho-Ser235).
Application
Western blot analysis of lysed extracts from PC12 cells untreated or treated with anisomycin using S6 Ribosomal Protein (Phospho-Ser235).| Application Notes | Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
