Application
Western blot analysis of SAE1 in SK-N-SH lysate with SAE1 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | SAE1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SAE1 Antibody: AOS1, SUA1, UBLE1A, HSPC140, AOS1, SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A |
| Product description | SAE1 Antibody: Small ubiquitin-like modifiers (SUMOs) are a family of small, related proteins (SUMO-1/2/3/4) that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation, a major regulator of protein function in cellular processes such as nuclear transport, transcriptional regulation, apoptosis and protein stability. This sumoylation is effected by the heterodimeric enzyme SAE1/SAE2 and the SUMO-1-conjugating enzyme Ubch9. The sumoylation pathway mediated by SAE1/SAE2 is distinct from other ubiquitin-like protein (Ubl) pathways.1) Kamitani T, Kito K, Nguyen HP, et al. Characterization of a second member of the sentrin family of ubiquitin-like proteins. J. Biol. Chem.1998;273:11349-53. |
| Immunogen | SAE1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human SAE1. The immunogen is located within amino acids 240 - 290 of SAE1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SAE1 antibody can be used for detection of SAE1 by Western blot at 1 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SAE1 |
|---|---|
| Alternative Names | SAE1 Antibody: AOS1 SUA1 UBLE1A HSPC140 AOS1 SUMO-activating enzyme subunit 1 Ubiquitin-like 1-activating enzyme E1A |
| Molecular Weight(MW) | Predicted: 38 kDa Observed: 39 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (90%) |
Application
Western blot analysis of SAE1 in SK-N-SH lysate with SAE1 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | SAE1 antibody can be used for detection of SAE1 by Western blot at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SAE1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SAE1 Antibody is supplied in PBS containing 0.02% sodium azide. |
