Application
Immunohistochemistry of SAMHD1 in human brain tissue with SAMHD1 antibody at 5 μg/ml.Application
Immunofluorescence of SAMHD1 in Daudi cells with SAMHD1 antibody at 20 μg/ml.Application
Western blot analysis of SAMHD1 in Daudi cell lysate with SAMHD1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | SAMHD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SAM domain and HD domain 1, DCIP, CHBL2, HDDC1, MOP-5, SBBI88 |
| Product description | The SAM domain and HD domain 1 (SAMHD1) protein is upregulated in response to viral infection and is thought to play a role in innate immunity (1). SAMHD1 blocks the infection of HIV-1 and SIVdeltaVpx before reverse transcription in macrophages and dendritic cells (2), and this restriction is regulated by phosphorylation of SAMHD1 (3). Mutations in this gene have been associated with Aicardi-Goutieres syndrome (1).1) Rice GI, Bond J, Asipu A, et al. Mutations involved in Aicardi-Goutieres syndrome implicate SAMHD1 as regulator of the innate immune system. Nat. Genet. 2009; 41:829-32. |
| Immunogen | SAMHD1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human SAMHD1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| SAMHD1 antibody can be used for detection of SAMHD1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SAMHD1 |
|---|---|
| Alternative Names | SAM domain and HD domain 1 DCIP CHBL2 HDDC1 MOP-5 SBBI88 |
| Molecular Weight(MW) | Predicted: 65, 69 kDa Observed: 62 kDa |
| Tissue Specificity | SAMHD1 antibody is human and mouse reactive. |
Application
Immunohistochemistry of SAMHD1 in human brain tissue with SAMHD1 antibody at 5 μg/ml.
Application
Immunofluorescence of SAMHD1 in Daudi cells with SAMHD1 antibody at 20 μg/ml.
Application
Western blot analysis of SAMHD1 in Daudi cell lysate with SAMHD1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | SAMHD1 antibody can be used for detection of SAMHD1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SAMHD1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | SAMHD1 antibody is supplied in PBS containing 0.02% sodium azide. |
