Application
Immunocytochemistry of SAMSN1 in HeLa cells with SAMSN1 antibody at 2.5 μg/ml.Application
Immunofluorescence of SAMSN1 in HeLa cells with SAMSN1 antibody at 5 μg/ml.Application
Western blot analysis of SAMSN in Hela cell lysate with SAMSN antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | SAMSN Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SAMSN Antibody: SLy2, HACS1, NASH1, SASH2, SH3D6B, SAM domain-containing protein SAMSN-1, Hematopoietic adaptor containing SH3 and SAM domains 1 |
| Product description | SAMSN Antibody: SAMSN (HACS1) is a member of a novel gene family of putative adaptors and scaffold proteins containing SH3 and SAM (sterile alpha motif) domains. It encodes a 441 amino acid protein that is differentially expressed in hematopoietic cells and malignancies including myeloid leukemia, lymphoma, and myeloma. SAMSN has restricted expression in human tissues. It has been shown to be up-regulated by B cell activation signals and is a participant in B cell activation and differentiation. SAMSN is an immunoinhibitory adaptor that might be a useful target for immune suppression therapy.1) Uchida T, Nakao A, Nakano N, et al. Identification of Nash1, a novel protein containing a nuclear localization signal, a sterile alpha motif, and an SH3 domain preferentially expressed in mast cells. Biochem. Biophys. Res. Commun. 2001; 288:137-41. |
| Immunogen | Rabbit polyclonal SAMSN antibody was raised against an 18 amino acid peptide near the carboxy terminus of human SAMSN. The immunogen is located within the last 50 amino acids of SAMSN. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| SAMSN antibody can be used for detection of SAMSN by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SAMSN1 |
|---|---|
| Alternative Names | SAMSN Antibody: SLy2 HACS1 NASH1 SASH2 SH3D6B SAM domain-containing protein SAMSN-1 Hematopoietic adaptor containing SH3 and SAM domains 1 |
| Molecular Weight(MW) | 41 kDa |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | At least two isoforms of SAMSN are known to exist. |
Application
Immunocytochemistry of SAMSN1 in HeLa cells with SAMSN1 antibody at 2.5 μg/ml.
Application
Immunofluorescence of SAMSN1 in HeLa cells with SAMSN1 antibody at 5 μg/ml.
Application
Western blot analysis of SAMSN in Hela cell lysate with SAMSN antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | SAMSN antibody can be used for detection of SAMSN by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SAMSN antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SAMSN Antibody is supplied in PBS containing 0.02% sodium azide. |
