SAP30BP expressed as a fusion polypeptide with a fluorescent protein in the nucleus of HeLa cells. SAP30BP induces cell death. The interaction of SAP30BP with SAP30 in its conserved domain implies that this protein family, as the products of immediate-early genes, comprise functional molecules involved in the transcriptional regulation of cells, which might be related to the inhibition of some cell survival genes. SAP30BP may also be involved in the regulation of beta-2-microglobulin genes.1) Li, J.F., et al., (2004) J. Biochem. 136 (2), 169-176.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SAP30BP.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
SAP30BP antibody can be used for detection of SAP30BP by ELISA at 1:12500. SAP30BP antibody can be used for detection of SAP30BP by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1219 - MCF7 Cell Lysate
Application Notes
SAP30BP antibody can be used for detection of SAP30BP by ELISA at 1:12500. SAP30BP antibody can be used for detection of SAP30BP by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SAP30BP antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.