Application
Immunofluorescence of SATB1 in Human Brain cells with SATB1 antibody at 20 μg/mL.Application
Immunohistochemistry of SATB1 in human brain with SATB1 antibody at 5 μg/mL.Application
Western blot analysis of SATB1 in A20 cell lysate with SATB1 antibody at (A) 2 and (B) 4 μg/mL.| Product Name | SATB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SATB1 Antibody: DNA-binding protein SATB1, Special AT-rich sequence-binding protein 1 |
| Product description | SATB1 Antibody: Human special AT-rich sequence-binding protein-1 (SATB1) is a nuclear matrix/scaffold-associated region DNA-binding protein, predominantly expressed in the thymus and pre-B cells. Like its homolog SATB2, SATB1 selectively binds double-stranded, special AT-rich DNA sequences in which one strand exclusively consists of well-mixed A, T, and C nucleotides. SATB1 contains a dimerization domain that shares similarity with the PDZ motif, identified as an indispensable element for high-affinity binding of SATB1 to DNA. SATB1 constitutes a functional nuclear architecture that has a 'cage-like' protein distribution surrounding heterochromatin and regulates gene expression through chromatin remodeling/HDAC (histone deacetylase complex) and transcription factors recruitment. SATB1 functions as a 'genome organizer' essential for proper T-cell development. Recent studies show that SATB1 is necessary for breast cancer cells to become metastatic, and when ectopically expressed in non-metastatic cells, can induce invasive activity in vivo. At least two isoforms of SATB1 are known to exist.1) Dickinson LA and Kohwi-Shigematsu T. Nucleolin is a matrix attachment region DNA-binding protein that specifically recognizes a region with high base-unpairing potential. Mol. Cell. Biol.1995; 15:456-65. |
| Immunogen | SATB1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human SATB1. The immunogen is located within amino acids 620 - 670 of SATB1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| SATB1 antibody can be used for detection of SATB1 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SATB1 |
|---|---|
| Alternative Names | SATB1 Antibody: DNA-binding protein SATB1 Special AT-rich sequence-binding protein 1 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunofluorescence of SATB1 in Human Brain cells with SATB1 antibody at 20 μg/mL.
Application
Immunohistochemistry of SATB1 in human brain with SATB1 antibody at 5 μg/mL.
Application
Western blot analysis of SATB1 in A20 cell lysate with SATB1 antibody at (A) 2 and (B) 4 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | SATB1 antibody can be used for detection of SATB1 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SATB1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SATB1 Antibody is supplied in PBS containing 0.02% sodium azide. |
