Application
Western blot analysis of SATB2 in A20 cell lysate with SATB2 antibody at (A) 2 and (B) 4 μg/mL.Application
Immunofluorescence of SATB2 in Human Brain cells with SATB2 antibody at 50 μg/mL.Application
Immunohistochemistry of SATB2 in human brain with SATB2 antibody at 5 μg/mL.| Product Name | SATB2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SATB2 Antibody: KIAA1034, DNA-binding protein SATB2, Special AT-rich sequence-binding protein 2 |
| Product description | SATB2 Antibody: Human special AT-rich sequence-binding protein-2 (SATB2) is a nuclear matrix/scaffold-associated region DNA-binding protein. Like its homolog SATB1, SATB2 selectively binds double-stranded, special AT-rich DNA sequences, but is expressed primarily in a subset of postmitotic, differentiating neurons in the neocortex. Mice deficient in SATB exhibit craniofacial abnormalities and defects in osteoblast differentiation and function. SATB2 also interacts with and enhances the activity of Runx2 and ATF4, two transcription factors that regulate osteoblast differentiation, indicating that SATB2 acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation. Recent experiments have shown that SATB2 interacts with histone deacetylase 1 and metastasis-associated protein 2, two proteins that are involved in chromatin remodeling, suggesting that SATB2 may also be involved in mediating epigenetic influences during cortical development.1) Dickinson LA and Kohwi-Shigematsu T. Nucleolin is a matrix attachment region DNA-binding protein that specifically recognizes a region with high base-unpairing potential. Mol. Cell. Biol.1995; 15:456-65. |
| Immunogen | SATB2 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of the human SATB2. The immunogen is located within amino acids 570 - 620 of SATB2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SATB2 antibody can be used for detection of SATB2 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SATB2 |
|---|---|
| Alternative Names | SATB2 Antibody: KIAA1034 DNA-binding protein SATB2 Special AT-rich sequence-binding protein 2 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | At least two isoforms of SATB2 are known to exist. This SATB2 antibody will not cross-react with SATB1. |
Application
Western blot analysis of SATB2 in A20 cell lysate with SATB2 antibody at (A) 2 and (B) 4 μg/mL.
Application
Immunofluorescence of SATB2 in Human Brain cells with SATB2 antibody at 50 μg/mL.
Application
Immunohistochemistry of SATB2 in human brain with SATB2 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | SATB2 antibody can be used for detection of SATB2 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SATB2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SATB2 Antibody is supplied in PBS containing 0.02% sodium azide. |
