Application
Immunofluorescence of SCARB1 in Human Spleen tissue with SCARB1 antibody at 20 μg/mL.Application
Immunohistochemistry of SCARB1 in human spleen tissue with SCARB1 antibody at 2.5 μg/mL.Application
Western blot analysis of SCARB1 in human spleen tissue lysate with SCARB1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | SCARB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SCARB1 Antibody: CLA1, SRB1, CLA-1, SR-BI, CD36L1, HDLQTL6, CLA1, Scavenger receptor class B member 1, CD36 and LIMPII analogous 1 |
| Product description | SCARB1 Antibody: Scavenger receptor class B member 1 (SCARB1), also known as SR-BI, is part of the scavenger receptor superfamily, which is composed of many members with diverse structures, expression patterns, and functions. SCARB1 is a multi-ligand cell-surface receptor that mediates the selective uptake of lipid from HDL cholesterol into cells and is expressed in steroidogenic tissues in adult animals. Other ligands of SCARB1 include native, acetylated, or oxidized LDL and anionic phospholipids. SCARB1-deficient mice have elevated HDL levels and increased susceptibility to atherosclerosis on fat feeding, indicating its importance in the regulation of cholesterol homeostasis. Along with CLDN1, LDL-R, and the tetraspanin superfamily member CD81, SCARB1 has been reported to be an entry factor for the Hepatitis C virus.1) Greaves DR, Gough PJ, and Gordon S. Recent progress in defining the role of scavenger receptors in lipid transport, atherosclerosis and host defence. Curr. Op. Lipid. 1998; 9:425-32. |
| Immunogen | SCARB1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human SCARB1. The immunogen is located within amino acids 70 - 120 of SCARB1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SCARB1 antibody can be used for detection of SCARB1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SCARB1 |
|---|---|
| Alternative Names | SCARB1 Antibody: CLA1 SRB1 CLA-1 SR-BI CD36L1 HDLQTL6 CLA1 Scavenger receptor class B member 1 CD36 and LIMPII analogous 1 |
| Molecular Weight(MW) | Predicted: 56, 60 kDa Observed: 60 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | At least two isoforms of SCARB1 are known to exist; this antibody can detect both isoforms. |
Application
Immunofluorescence of SCARB1 in Human Spleen tissue with SCARB1 antibody at 20 μg/mL.
Application
Immunohistochemistry of SCARB1 in human spleen tissue with SCARB1 antibody at 2.5 μg/mL.
Application
Western blot analysis of SCARB1 in human spleen tissue lysate with SCARB1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1306 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | SCARB1 antibody can be used for detection of SCARB1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SCARB1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SCARB1 Antibody is supplied in PBS containing 0.02% sodium azide. |
