Application
Immunohistochemical staining of human brain tissue using Scrapper antibody at 2.5 μg/mL.Application
Immunofluorescence of scrapper in human brain tissue with scrapper antibody at 20 μg/mL.Application
Western blot analysis of SCRAPPER in A20 cell lysate with SCRAPPER antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | SCRAPPER Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SCRAPPER Antibody: Fbl2, Fbl20, FBL2, F-box and leucine-rich repeat protein 20 |
| Product description | SCRAPPER Antibody: Members of the F-box protein family, such as Scrapper, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1, cullin, and F-box proteins, act as protein-ubiquitin ligases. Scrapper is selectively expressed in the brain, broadly expressed within the mouse CNS and is abundant at presynaptic membrane. Scrapper has orthologs in C. elegans, D. melanogaster, and mammals which suggests that it might function as an important membrane-localized E3 ligase in various species. Scrapper is a major presynaptic E3 ubiquitin ligase that acts through RIM1a via degradation and the ubiquitin-proteasome-system (UPS) pathway to critically regulate synaptic transmission. This identifies protein degradation as a mechanism for holding synaptic communication in check.1) Ho MS, Tsai PI, and Chien CT. F-box proteins: the key to protein degradation. J. Biomed. Sci.2006; 13:181-91. |
| Immunogen | SCRAPPER antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of the human SCRAPPER. The immunogen is located within the first 50 amino acids of SCRAPPER. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SCRAPPER antibody can be used for detection of SCRAPPER by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FBXL20 |
|---|---|
| Alternative Names | SCRAPPER Antibody: Fbl2 Fbl20 FBL2 F-box and leucine-rich repeat protein 20 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemical staining of human brain tissue using Scrapper antibody at 2.5 μg/mL.
Application
Immunofluorescence of scrapper in human brain tissue with scrapper antibody at 20 μg/mL.
Application
Western blot analysis of SCRAPPER in A20 cell lysate with SCRAPPER antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | SCRAPPER antibody can be used for detection of SCRAPPER by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SCRAPPER antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SCRAPPER Antibody is supplied in PBS containing 0.02% sodium azide. |
