Application
Western blot analysis in Hela and mouse C2C12 cell line lysates (35ug/lane).Application
Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using SDHA Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.| Product Name | SDHA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor.1) Hirawake H., et al. J. Biochem. 116:221-227(1994). |
| Immunogen | This SDHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 577-605 amino acids from the C-terminal region of human SDHA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SDHA |
|---|---|
| Alternative Names | Succinate dehydrogenase [ubiquinone] flavoprotein subunit mitochondrial Flavoprotein subunit of complex II Fp SDHA SDH2 SDHF |
| Molecular Weight(MW) | 73 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Xenopus |
Application
Western blot analysis in Hela and mouse C2C12 cell line lysates (35ug/lane).
Application
Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using SDHA Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.| Application Notes | For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
