SEC14L2 encodes a cytosolic protein which belongs to a family of lipid-binding proteins including Sec14p, alpha-tocopherol transfer protein, and cellular retinol-binding protein. The encoded protein stimulates squalene monooxygenase which is a downstream enzyme in the cholesterol biosynthetic pathway.1) Collins, J.E., et al., (2004) Genome Biol. 5(10), R84.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SEC14L2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
SEC14L2 antibody can be used for detection of SEC14L2 by ELISA at 1:7812500. SEC14L2 antibody can be used for detection of SEC14L2 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human Liver cell lysates at 0.2-1 ug/ml.
Positive Control
1) Cat. No. XBL-10409 - Fetal Liver Tissue Lysate
Application Notes
SEC14L2 antibody can be used for detection of SEC14L2 by ELISA at 1:7812500. SEC14L2 antibody can be used for detection of SEC14L2 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SEC14L2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.