Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SEMA3A antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of SEMA3A Antibody in CEM cell line lysates (35ug/lane)| Product Name | SEMA3A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SEMA3A is a member of the semaphorin family with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease.1) Narazaki,M., Blood 111 (8), 4126-4136 (2008) |
| Immunogen | This SEMA3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 535-566 amino acids from the Central region of human SEMA3A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | SEMA3A |
|---|---|
| Alternative Names | Semaphorin-3A Semaphorin III Sema III SEMA3A SEMAD |
| Molecular Weight(MW) | 89 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken |
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SEMA3A antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of SEMA3A Antibody in CEM cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM290828