Alpha-1-antitrypsin is a protease inhibitor, deficiency of which is associated with emphysema and liver disease. The protein is encoded by a gene (PI) located on the distal long arm of chromosome 14.1) Knappstein, S., et al., (2004) Infect. Immun. 72 (8), 4344-4350.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SERPINA1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
SERPINA1 antibody can be used for detection of SERPINA1 by ELISA at 1:12500. SERPINA1 antibody can be used for detection of SERPINA1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human HepG2 cells at 1.25 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
SERPINA1 antibody can be used for detection of SERPINA1 by ELISA at 1:12500. SERPINA1 antibody can be used for detection of SERPINA1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SERPINA1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt