SERTA is a transcriptional regulator that interacts with the PHD-bromodomain of co-repressors of Kruppel-associated box (KRAB)-mediated repression, KRIP-1 (TIF1beta) and TIF1alpha, as well as the co-activator/adaptor p300/CBP. It is a component of a multiprotein complex containing E2F-1 and DP-1.1) Li, J., (2005) Biochemistry 44 (40), 13246-13256.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SERTAD1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
SERTAD1 antibody can be used for detection of SERTAD1 by ELISA at 1:312500. SERTAD1 antibody can be used for detection of SERTAD1 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human Placenta at 0.125 ug/ml.
Application
Antibody used in IHC on Human Muscle.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
SERTAD1 antibody can be used for detection of SERTAD1 by ELISA at 1:312500. SERTAD1 antibody can be used for detection of SERTAD1 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SERTAD1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt