Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of SET07 polyclonal antibody in HeLa cell lysate (Lane 1) and NIH/3T3 cell lysate (Lane 2)| Product Name | SET07 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SET07 is a histone methyltransferase that methylates Lys-20 of histone H4. H4 Lys-20 methylation represents a specific tag for epigenetic transcriptional repression. The nuclear SET07 protein, which associates with silent chromatin on euchromatic arms but shows no association with constitutive heterochromatin, prefers nucleosomes as substrate compared to free histones. It appears that SET07 may play a role in maintaining silent chromatin by preventing neighboring acetylation of H4 tail. Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity.1) Nishioka, K., et al., Mol. Cell 9(6):1201-1213 (2002). |
| Immunogen | This SET07 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-53 amino acids from the N-terminal region of human SET07. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | SETD8 |
|---|---|
| Alternative Names | N-lysine methyltransferase SETD8 211- H4-K20-HMTase SETD8 Histone-lysine N-methyltransferase SETD8 Lysine N-methyltransferase 5A PR/SET domain-containing protein 07 PR-Set7 PR/SET07 SET domain-containing protein 8 SETD8 KMT5A PRSET7 SET07 SET8 |
| Molecular Weight(MW) | 43 kDa |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of SET07 polyclonal antibody in HeLa cell lysate (Lane 1) and NIH/3T3 cell lysate (Lane 2)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
