Application
Western blot analysis in MCF-7 cell line lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of SET9 using rabbit polyclonal SET9 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SET9 gene (Lane 2).Application
Antibody is used in Western blot to detect SET9 in mouse brain tissue lysate.| Product Name | SET7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Similar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. The retinoblastoma protein-interacting zinc finger geneRIZ1 is a tumor suppressor gene and a FOUNDING member of the PR domain family. RIZ1 inactivation is commonly found in many types of human cancers and occurs through loss of mRNA expression, frame shift mutation, chromosomal deletion, and missense mutation. RIZ1 is also a tumor susceptibility gene in mice. The loss of RIZ1 mRNA in human cancers was shown to associate with DNA methylation of its promoter CpG island. Methylation of the RIZ1 promoter strongly correlated with lost or decreased RIZ1 mRNA expression in breast, liver, colon, and lung cancer cell lines as well as in liver cancer tissues.1) Wysocka, J., et al., Genes Dev. 17(7):896-911 (2003). Xiao, B., et al., Nature 421(6923):652-656 (2003). Kwon, T., et al., EMBO J. 22(2):292-303 (2003). Nishioka, K., et al., Genes Dev. 16(4):479-489 (2002). Wilson, J.R., et al., Cell 111(1):105-115 (2002). |
| Immunogen | This SET7 (SET9) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-189 amino acids from the Central region of human SET7 (SET9). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SETD7 |
|---|---|
| Alternative Names | Histone-lysine N-methyltransferase SETD7 Histone H3-K4 methyltransferase SETD7 H3-K4-HMTase SETD7 Lysine N-methyltransferase 7 SET domain-containing protein 7 SET7/9 SETD7 KIAA1717 KMT7 SET7 SET9 |
| Molecular Weight(MW) | 41 kDa |
Application
Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of SET9 using rabbit polyclonal SET9 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SET9 gene (Lane 2).
Application
Antibody is used in Western blot to detect SET9 in mouse brain tissue lysate.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
