Application
Antibody used in WB on Human Pancreas at 5.0 ug/ml.| Product Name | SETD7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SETD7 is a histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. It plays a central role in the transcriptional activation of genes such as collagenase or insulin. It is recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. It has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins.1) Subramanian, K., (2008) Mol. Cell 30 (3), 336-347. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SETD7. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SETD7 antibody can be used for detection of SETD7 by ELISA at 1:312500. SETD7 antibody can be used for detection of SETD7 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SETD7 |
|---|---|
| Alternative Names | SETD7 FLJ21193 KIAA1717 KMT7 SET7 SET7/9 SET9 |
| Molecular Weight(MW) | 41 kDa |
Application
Antibody used in WB on Human Pancreas at 5.0 ug/ml.| Positive Control | 1) Fetal Pancreas Lysate |
|---|---|
| Application Notes | SETD7 antibody can be used for detection of SETD7 by ELISA at 1:312500. SETD7 antibody can be used for detection of SETD7 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SETD7 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
