Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using SF1 (Phospho-Ser82) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from HUVEC cells treated with anisomycin using SF1 (Phospho-Ser82) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | SF1 (phospho Ser82) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.1) Susan Y. Park, Development, May 2005; 132: 2415 - 2423. |
| Immunogen | SF1 (phospho Ser82) antibody was raised against a peptide sequence around phosphorylation site of Serine 82 (S-P-S (p)-P-E) derived from Human SF1. |
| Modification | p-Ser82 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SF1 |
|---|---|
| Alternative Names | BBP CW17 SF01 ZFM1 ZNF162 |
| Molecular Weight(MW) | 68 kDa |
| Tissue Specificity | The antibody detects endogenous levels of SF1 only when phosphorylated at serine 82. |
| Entrez Gene | 7536 |
|---|---|
| Protein Accession | NP_004621.2 |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using SF1 (Phospho-Ser82) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from HUVEC cells treated with anisomycin using SF1 (Phospho-Ser82) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
