Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Product Name | SF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SF1 contains 1 CCHC-type zinc finger and 1 KH domain. SF1 is Necessary for the ATP-dependent first step of spliceosome assembly. It binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. SF1 may act as transcription repressor.This gene encodes a member of the heat shock factor (HSF) family of transcriptional activators for heat shock proteins. This gene is a candidate gene for azoospermia, since it localizes to a region of chromosome Y that is sometimes deleted in infertile males. The genome has two identical copies of this gene within a palindromic region; this record represents the more centromeric copy. Alternative splicing results in multiple transcript variants encoding distinct isoforms.1) Rino, J., (2008) Mol. Cell. Biol. 28 (9), 3045-3057. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SF1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SF1 antibody can be used for detection of SF1 by ELISA at 1:12500. SF1 antibody can be used for detection of SF1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SF1 |
|---|---|
| Alternative Names | SF1 D11S636 ZFM1 ZNF162 BBP MBBP ZCCHC25 |
| Molecular Weight(MW) | 69 kDa |
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | SF1 antibody can be used for detection of SF1 by ELISA at 1:12500. SF1 antibody can be used for detection of SF1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SF1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
