Application
Antibody used in WB on Human Thymus at 1.25 ug/ml.Application
Antibody used in IHC on Human Liver at 4.0-8.0 ug/ml.Application
Antibody used in IHC on Human Skin at 16 ug/ml.| Product Name | SF3B1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SF3B1 is subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures.This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.1) Beausoleil, S.A., (2004) Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12130-12135. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SF3B1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| SF3B1 antibody can be used for detection of SF3B1 by ELISA at 1:62500. SF3B1 antibody can be used for detection of SF3B1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SF3B1 |
|---|---|
| Alternative Names | SF3B1 PRP10 SAP155 SF3b155 MDS Hsh155 PRPF10 |
| Molecular Weight(MW) | 143 kDa, 16 kDa |
Application
Antibody used in WB on Human Thymus at 1.25 ug/ml.
Application
Antibody used in IHC on Human Liver at 4.0-8.0 ug/ml.
Application
Antibody used in IHC on Human Skin at 16 ug/ml.| Positive Control | 1) Cat. No. XBL-10426 - Fetal Thymus Tissue Lysate |
|---|---|
| Application Notes | SF3B1 antibody can be used for detection of SF3B1 by ELISA at 1:62500. SF3B1 antibody can be used for detection of SF3B1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SF3B1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
Data sheet for OM290990