Application
Antibody used in WB on Human Placenta at 0.2-1 ug/ml.| Product Name | SF3B14 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SF3B14 is a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. SF3B14 also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site.This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site.1) Ewing, R.M., Mol. Syst. Biol. 3, 89 (2007). |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SF3B14. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SF3B14 antibody can be used for detection of SF3B14 by ELISA at 1:62500. SF3B14 antibody can be used for detection of SF3B14 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SF3B14 |
|---|---|
| Alternative Names | SF3B14 CGI-110 HSPC175 Ht006 P14 SAP14 SF3B14a SAP14a SF3B14 |
| Molecular Weight(MW) | 14 kDa |
Application
Antibody used in WB on Human Placenta at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1309 - Human Placenta Lysate |
|---|---|
| Application Notes | SF3B14 antibody can be used for detection of SF3B14 by ELISA at 1:62500. SF3B14 antibody can be used for detection of SF3B14 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SF3B14 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
