Application
Antibody used in IHC on Human Skin at 4.0-8.0 ug/ml.Application
Antibody used in WB on Human HeLa cells at: 1:1000.Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.Application
Antibody used in IHC on Human Intestine at 4.0-8.0 ug/ml.| Product Name | SFPQ Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SFPQ is DNA- and RNA binding protein. It is essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. It binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. It interacts with U5 snRNA. It may be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. SFPQ may be involved in homologous DNA pairing. SFPQ is involved in transcriptional regulation. It binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.1) Rosonina, E., (2005) Mol. Cell. Biol. 25 (15), 6734-6746. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SFPQ. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| SFPQ antibody can be used for detection of SFPQ by ELISA at 1:12500. SFPQ antibody can be used for detection of SFPQ by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SFPQ |
|---|---|
| Alternative Names | SFPQ PSF POMP100 |
| Molecular Weight(MW) | 78 kDa |
Application
Antibody used in IHC on Human Skin at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Human HeLa cells at: 1:1000.
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Application
Antibody used in IHC on Human Intestine at 4.0-8.0 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | SFPQ antibody can be used for detection of SFPQ by ELISA at 1:12500. SFPQ antibody can be used for detection of SFPQ by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SFPQ antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
