Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.| Product Name | SGMS2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SGMS2 is a bidirectional lipid cholinephosphotransferase capable of converting phosphatidylcholine (PC) and ceramide to sphingomyelin (SM) and diacylglycerol (DAG) and vice versa. Direction is dependent on the relative concentrations of DAG and ceramide as phosphocholine acceptors. SGMS2 directly and specifically recognizes the choline head group on the substrate. SGMS2 also requires two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. SGMS2 does not function strictly as a SM synthase. SGMS2 is required for cell growth.Sphingomyelin (SM) is a major component of plasma membranes. It is preferentially concentrated in the outer leaflet and has a role in the formation of lipid rafts. SM synthases (EC 2.7.8.27), such as SGMS2, produce SM in the lumen of the Golgi and on the cell surface through the transfer of phosphocholine from phosphatidylcholine onto ceramide, yielding diacylglycerol as a side product (Huitema et al., 2004 [PubMed 14685263]).1) Ding, T., (2008) J. Lipid Res. 49 (2), 376-385. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SGMS2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SGMS2 antibody can be used for detection of SGMS2 by ELISA at 1:1562500. SGMS2 antibody can be used for detection of SGMS2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SGMS2 |
|---|---|
| Alternative Names | SGMS2 MGC26963 SMS2 |
| Molecular Weight(MW) | 42 kDa |
Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.| Positive Control | 1) 293T Cell Lysate |
|---|---|
| Application Notes | SGMS2 antibody can be used for detection of SGMS2 by ELISA at 1:1562500. SGMS2 antibody can be used for detection of SGMS2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SGMS2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
