Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SHP-2 (Ab-542). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using SHP-2 (Ab-542).Application
Western blot analysis of lysed extracts from 3T3 cells using SHP-2 (Ab-542).| Product Name | SHP 2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.1) Ferjoux G, et al. (2003) Mol Biol Cell. 2003; 14 (9): 3911-3928. Shi ZQ, et al. (2000) Mol Cell Biol ; 20 (5): 1526-1536. Li C, Friedman JM. (1999) Proc Natl Acad Sci U S A ; 96 (17): 9677-9682Manes S, et al. (1999) Mol Cell Biol ; 19 (4): 3125-3135. Oh ES, et al. (1999) Mol Cell Biol; 19 (4): 3205-3215. |
| Immunogen | SHP-2 (Ab-542) antibody was raised against a peptide sequence around aa. 540~544 (H-E-Y-T-N) derived from Human SHP-2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTPN11 |
|---|---|
| Alternative Names | CFC NS1 SHP2 BPTP3 PTP2C PTP-1D SH-PTP2 SH-PTP3 ERBB ERBB1 HER1 Protein-tyrosine phosphatase 2C |
| Molecular Weight(MW) | 72 kDa |
| Tissue Specificity | This antibody detects endogenous level of total SHP-2 protein. |
| Entrez Gene | 5781 |
|---|---|
| Protein Accession | NP_002825.3 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SHP-2 (Ab-542). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using SHP-2 (Ab-542).
Application
Western blot analysis of lysed extracts from 3T3 cells using SHP-2 (Ab-542).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
