Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SHP-2 (Phospho-Tyr542).Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with starvation using SHP-2 (Phospho-Tyr542).| Product Name | SHP 2 (phospho Tyr542) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.1) Ferjoux G, et al. (2003) Mol Biol Cell. 2003; 14 (9): 3911-3928. Shi ZQ, et al. (2000) Mol Cell Biol. 20 (5): 1526-1536. Li C, Friedman JM. (1999) Proc Natl Acad Sci U S A ; 96 (17): 9677-9682Manes S, et al. (1999) Mol Cell Biol. 19 (4): 3125-3135. Oh ES, et al. (1999) Mol Cell Biol; 19 (4): 3205-3215. |
| Immunogen | SHP-2 (Phospho-Tyr542) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 542 (H-E-Y (p) -T-N) derived from Human SHP-2. |
| Modification | p-Tyr542 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTPN11 |
|---|---|
| Alternative Names | CFC NS1 SHP2 BPTP3 PTP2C PTP-1D SH-PTP2 SH-PTP3 FRAP FRAP1 FRAP2 RAFT1 RAPT1 PTN11 PTP-2C PTPN11 |
| Molecular Weight(MW) | 72 kDa |
| Tissue Specificity | This antibody detects endogenous level of SHP-2 only when phosphorylated at tyrosine 542. |
| Entrez Gene | 5781 |
|---|---|
| Protein Accession | NP_002825.3 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SHP-2 (Phospho-Tyr542).
Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with starvation using SHP-2 (Phospho-Tyr542).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
