Application
Antibody used in WB on Human HEK293T cells at: 1:1000.Application
Antibody used in WB on Human Placenta at 1.25 ug/ml.Application
Antibody used in IHC on Human Liver.| Product Name | SIAH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SIAH1 is a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterizedThis gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.1) Avraham, E., et al., (2005) J. Biol. Chem. 280 (52), 42877-42886. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SIAH1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SIAH1 antibody can be used for detection of SIAH1 by ELISA at 1:312500. SIAH1 antibody can be used for detection of SIAH1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SIAH1 |
|---|---|
| Alternative Names | SIAH1 SIAH1A |
| Molecular Weight(MW) | 31 kDa |
Application
Antibody used in WB on Human HEK293T cells at: 1:1000.
Application
Antibody used in WB on Human Placenta at 1.25 ug/ml.
Application
Antibody used in IHC on Human Liver.| Positive Control | 1) Cat. No. 1309 - Human Placenta Lysate |
|---|---|
| Application Notes | SIAH1 antibody can be used for detection of SIAH1 by ELISA at 1:312500. SIAH1 antibody can be used for detection of SIAH1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SIAH1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
