Application
Formalin-fixed and paraffin-embedded human spleen tissue reacted with SIGLEC12 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of SIGLEC12 Antibody in mouse bladder tissue lysates (35ug/lane)| Product Name | SIGLEC12 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. SIGLEC12 is a member of the SIGLEC3-like subfamily of SIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like. This protein, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor.1) Angata,T., J. Biol. Chem. 276 (43), 40282-40287 (2001) |
| Immunogen | This SIGLEC12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 565-595 amino acids from the C-terminal region of human SIGLEC12. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | SIGLEC12 |
|---|---|
| Alternative Names | Sialic acid-binding Ig-like lectin 12 Siglec-12 Sialic acid-binding Ig-like lectin-like 1 Siglec-L1 SIGLEC12 SIGLECL1 SLG |
| Molecular Weight(MW) | 65 kDa |
Application
Formalin-fixed and paraffin-embedded human spleen tissue reacted with SIGLEC12 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of SIGLEC12 Antibody in mouse bladder tissue lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
