Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Antibody is used in Western blot to detect Siglec9 in mouse liver tissue lysate.Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).| Product Name | SIGLEC9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SIGLEC9 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. This protein is expressed by peripheral blood leukocytes (neutrophils and monocytes but not eosinophils). It is found in liver, fetal liver, bone marrow, placenta, spleen and in lower levels in skeletal muscle, fetal brain, stomach, lung, thymus, prostate, brain, mammary, adrenal gland, colon, trachea, cerebellum, testis, small intestine and spinal cordon. SIGLEC9 contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.1) Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003). Zhang, J.Q., et al., J. Biol. Chem. 275(29):22121-22126 (2000). Foussias, G., et al., Genomics 67(2):171-178 (2000). Angata, T., et al., J. Biol. Chem. 275(29):22127-22135 (2000). Yousef, G.M., et al., Anticancer Res. 19 (4B), 2843-2852 (1999). |
| Immunogen | This SIGLEC9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 385-415 amino acids from the C-terminal region of human SIGLEC9. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | SIGLEC9 |
|---|---|
| Alternative Names | Sialic acid-binding Ig-like lectin 9 Siglec-9 CDw329 Protein FOAP-9 CD329 SIGLEC9 |
| Molecular Weight(MW) | 50 kDa |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Antibody is used in Western blot to detect Siglec9 in mouse liver tissue lysate.
Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
