Application
Immunocytochemistry of SIK2 in SW480 cells with SIK2 antibody at 2.5 μg/ml.Application
Immunofluorescence of SIK2 in SW480 cells with SIK2 antibody at 5 μg/ml.Application
Western blot analysis of SIK2 in SW480 cell lysate with SIK2 antibody at 1 μg/mL.| Product Name | SIK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SIK2 Antibody: Salt-inducible kinase 2 (SIK2), like its closely related homolog SIK1, belongs AMPK subfamily of the Ser/Thr protein kinase family and negatively regulates CRE-binding protein (CREB) activity by phosphorylating the CREB-specific coactivator transducer of regulated CREB activity (TORC). SIK2 is thought to be part of a signaling cascade that regulates the expression and activity of the insulin-induced genes PGC-1 alpha and UCP-1 in brown adipocytes, impairment of which has been implicated in obesity and insulin resistance in human and animal models. SIK2 has also been reported as a key regulator for neuronal survival after ischemia, suppressing CREB-mediated gene expression after oxygen-glucose deprivation.1) Horike N, Takemori H, Katoh Y, et al. Adipose-specific expression, phosphorylation of Ser794 in insulin receptor substrate-1, and activation in diabetic animals of salt-inducible kinase-2. J. Biol. Chem. 2003; 278:18440-7. |
| Immunogen | SIK2 antibody was raised against a 16 amino acid synthetic peptide near the center of human SIK2. The immunogen is located within amino acids 270 - 320 of SIK2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| SIK2 antibody can be used for detection of SIK2 by Western blot at 1 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIK2 |
|---|---|
| Alternative Names | SIK2 Antibody: QIK SNF1LK2 LOH11CR1I KIAA0781 QIK Qin-induced kinase SIK-2 |
| Tissue Specificity | SIK2 antibody is predicted to not cross-react with other SIK protein family members. |
Application
Immunocytochemistry of SIK2 in SW480 cells with SIK2 antibody at 2.5 μg/ml.
Application
Immunofluorescence of SIK2 in SW480 cells with SIK2 antibody at 5 μg/ml.
Application
Western blot analysis of SIK2 in SW480 cell lysate with SIK2 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1221 - SW480 Cell Lysate |
|---|---|
| Application Notes | SIK2 antibody can be used for detection of SIK2 by Western blot at 1 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SIK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SIK2 Antibody is supplied in PBS containing 0.02% sodium azide. |
