SIL1 is a resident endoplasmic reticulum (ER), N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein. Mutations in its gene have been associated with Marinesco-Sjogren syndrome.This gene encodes a resident endoplasmic reticulum (ER), N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein. Mutations in this gene have been associated with Marinesco-Sjogren syndrome. Alternate transcriptional splice variants have been characterized.1) Karim, M.A., (2006) Clin. Genet. 70 (5), 420-423.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SIL1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
SIL1 antibody can be used for detection of SIL1 by ELISA at 1:312500. SIL1 antibody can be used for detection of SIL1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Heart at 1 ug/ml.
Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1201 - HeLa Cell Lysate
Application Notes
SIL1 antibody can be used for detection of SIL1 by ELISA at 1:312500. SIL1 antibody can be used for detection of SIL1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SIL1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.