Application
Antibody used in WB on Human HCT15 at 0.2-1 ug/ml.| Product Name | SIN3B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SIN3B acts as a transcriptional repressor. It interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also, it interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. The gene is also able to form a complex with FOXK1 which represses transcription1) Dhanda, R.S., (er) BMC Mol. Biol. 9, 8 (2008). |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SIN3B. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SIN3B antibody can be used for detection of SIN3B by ELISA at 1:62500. SIN3B antibody can be used for detection of SIN3B by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIN3B |
|---|---|
| Alternative Names | SIN3B KIAA0700 |
| Molecular Weight(MW) | 133 kDa |
Application
Antibody used in WB on Human HCT15 at 0.2-1 ug/ml.| Positive Control | 1) HCT15 Cell Lysate |
|---|---|
| Application Notes | SIN3B antibody can be used for detection of SIN3B by ELISA at 1:62500. SIN3B antibody can be used for detection of SIN3B by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SIN3B antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
