Application
Antibody used in WB on Human Thymus at 0.2-1 ug/ml.Application
Antibody used in IHC on Human kidney.| Product Name | SIRT3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The SIRT3 gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. SIRT3 encodes a protein that is included in class I of the sirtuin family.1) Onyango, P., et al., (2002) Proc. Natl. Acad. Sci. U.S.A. 15 99(21), 13653-8. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SIRT3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| SIRT3 antibody can be used for detection of SIRT3 by ELISA at 1:62500. SIRT3 antibody can be used for detection of SIRT3 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIRT3 |
|---|---|
| Alternative Names | SIRT3 SIR2L3 |
| Molecular Weight(MW) | 28 kDa, 44 kDa |
| Entrez Gene | 23410 |
|---|---|
| Protein Accession | NP_001017524 |
Application
Antibody used in WB on Human Thymus at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human kidney.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | SIRT3 antibody can be used for detection of SIRT3 by ELISA at 1:62500. SIRT3 antibody can be used for detection of SIRT3 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SIRT3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
