Application
Immunofluorescence of Ski in human kidney tissue with Ski antibody at 20 μg/mL.Application
Western blot analysis of Ski in HeLa cell lysate with Ski antibody at (A) 1 and (B) 2 μg/mL.| Product Name | Ski Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Ski Antibody: TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Ski functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. Ski will also bind to the Smad proteins specific to bone morphogenic proteins (BMPs) and block BMP signaling in mammalian cells.1) Derynck R, Akhurst RJ, and Balmain A. TGF-β signaling in tumor suppression and cancer progression. Nat. Genet. 2001; 29:117-129. |
| Immunogen | Ski antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human Ski. The immunogen is located within the first 50 amino acids of Ski. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| SkiP antibody can be used for detection of Ski by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SKI |
|---|---|
| Alternative Names | Ski Antibody: SGS SKV Ski oncogene Proto-oncogene c-Ski |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
Application
Immunofluorescence of Ski in human kidney tissue with Ski antibody at 20 μg/mL.
Application
Western blot analysis of Ski in HeLa cell lysate with Ski antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
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| Application Notes | SkiP antibody can be used for detection of Ski by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
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| Storage Instructions | Ski antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ski Antibody is supplied in PBS containing 0.02% sodium azide. |
