Application
Immunohistochemistry staining of SKIL in lung, respiratory epithelium tissue using SKIL Antibody.| Product Name | SKIL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SKIL, Ski-related oncogene, SnoI, SnoN, SKI-like oncogene, Sno, Ski-related oncogene snoN, Ski-related protein, Ski-like protein, SnoA |
| Product description | Principal Names: SnoN; SNO; SKI-like; Ski-related Oncogene; SnoA; SKIL Official Gene Symbol- SKIL Gen Bank Accession Number- NP_005405 Gene ID- 6498(Human) 20482 (mouse)Gene Map Locus- 3q26 (human) SnoN, an 80 kDa protein, is a member of ski family of nuclear proto-oncogenes involved in regulation of cellular transformation and differentiation. Primarily expressed in two isoforms, SnoN is localized in cytoplasm in normal tissues and non-tumorigenic primary epithelial cells. In cancer tissues or cells, SnoN is exclusively localized in the nucleus. It plays a vital role in inhibition of cell cycle arrest induced by TGF-. Upon morphological differentiation or cell-cycle arrest, SnoN translocates into the nucleus, binds to Smad2, Smad3, and Smad4 on TGF- -responsive promoters and represses their ability to activate expression of TGF- target genes. It has also been found to mediate transcriptional repression of thyroid hormone receptor, Mad and pRb. Increased expression of SnoN has been detected in many human tumor cell lines suggesting a clinical significance. |
| Immunogen | SKIL antibody was raised against amino acids 600 - 684 of SKIL (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| SKIL antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, and immunohistochemistry starting at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | SKIL |
|---|---|
| Alternative Names | SKIL Ski-related oncogene SnoI SnoN SKI-like oncogene Sno Ski-related oncogene snoN Ski-related protein Ski-like protein SnoA |
Application
Immunohistochemistry staining of SKIL in lung, respiratory epithelium tissue using SKIL Antibody.| Application Notes | SKIL antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, and immunohistochemistry starting at 2.5 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | SKIL antibody can be stored short term 4 ˚C. For long term storage aliquot and store at -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.2% gelatin, 0.05% sodium azide. |
