Application
Immunofluorescence of SLAIN1 in A549 cells with SLAIN1 antibody at 2.5 μg/mL.Application
Western blot analysis of SLAIN1 in A549 cell lysate with SLAIN1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | SLAIN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SLAIN1 Antibody: C13orf32, C13orf32, SLAIN motif-containing protein 1 |
| Product description | SLAIN1 Antibody: SLAIN1 is a novel stem cell protein that was identified by transciptional profiling of mouse and human embryonic stem cells (ESCs) and is expressed at the stem cell and epiblast stages. Unlike its more widely expressed homolog SLAIN2, SLAIN1 was found to be expressed in the epiblast, nervous system, tailbud and somites of the developing mouse embryo later experiments suggested that SLAIN1 may play a role in the development of the nervous system as well as in the morphogenesis of several embryonic structures.1) Hirst CE, Ng ES, Azzola L, et al. Transcriptional profiling of mouse and human ES cells identifies SLAIN1, a novel stem cell gene. Dev. Biol. 2006; 293:90-103. |
| Immunogen | SLAIN1 antibody was raised against an 15 amino acid synthetic peptide near the carboxy terminus of human SLAIN1. The immunogen is located within the last 50 amino acids of SLAIN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| SLAIN1 antibody can be used for detection of SLAIN1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLAIN1 |
|---|---|
| Alternative Names | SLAIN1 Antibody: C13orf32 C13orf32 SLAIN motif-containing protein 1 |
| Sequence Similarities | 2) Cat. No. 17-003 - A549 Cell Slide |
| Tissue Specificity | At least three isoforms of SLAIN1 are known to exist; this antibody will detect all three. SLAIN1 antibody is predicted not to cross-react with SLAIN2. |
Application
Immunofluorescence of SLAIN1 in A549 cells with SLAIN1 antibody at 2.5 μg/mL.
Application
Western blot analysis of SLAIN1 in A549 cell lysate with SLAIN1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1203 - A549 Cell Lysate |
|---|---|
| Application Notes | SLAIN1 antibody can be used for detection of SLAIN1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SLAIN1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SLAIN1 Antibody is supplied in PBS containing 0.02% sodium azide. |
