Application
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
SLC25A37 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in K562 cell line and rat spleen tissue lysates (35ug/lane).Application
Immunofluorescent analysis of Hela cells, using SLC25A37 Antibody . Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).| Product Name | SLC25A37 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Mitoferrin-1, Mitochondrial iron transporter 1, Mitochondrial solute carrier protein, Solute carrier family 25 member 37, SLC25A37, MFRN, MSCP |
| Product description | SLC25A37 belongs to the SLC25 family of mitochondrial carrier proteins (Haitina et al., 2006 [PubMed 16949250]).[supplied by OMIM].1) Kaneko, K., et al. FEBS J. 276(5):1370-1382(2009) Haitina, T., et al. Genomics 88(6):779-790(2006) Shaw, G.C., et al. Nature 440(7080):96-100(2006) Li, Q.Z., et al. Mamm. Genome 12(11):830-836(2001) |
| Immunogen | This SLC25A37 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-338 amino acids from the C-terminal region of human SLC25A37. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For IF starting dilution is: 1:25 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC25A37 |
|---|---|
| Alternative Names | Mitoferrin-1 Mitochondrial iron transporter 1 Mitochondrial solute carrier protein Solute carrier family 25 member 37 SLC25A37 MFRN MSCP |
| Molecular Weight(MW) | 37 kDa |
Application
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
SLC25A37 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in K562 cell line and rat spleen tissue lysates (35ug/lane).
Application
Immunofluorescent analysis of Hela cells, using SLC25A37 Antibody . Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).| Application Notes | For IF starting dilution is: 1:25 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
