Under hypertonic conditions the induction of SLC38A2/SNAT2 leads to the stimulation of transport system A and to the increase in the cell content of amino acids. Its amino acid response element, along with a nearby conserved CAAT box, has enhancer activity in that it functions in an orientation and position independent manner, and it confers regulated transcription to a heterologous promoter.1) Zhang, Z. (2007) Biophys. J. 92 (7), 2621-2632 .
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SLC38A2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
SLC38A2 antibody can be used for detection of SLC38A2 by ELISA at 1:1562500. SLC38A2 antibody can be used for detection of SLC38A2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HT1080 cells at 0.2-1 ug/ml.
Positive Control
1) HT1080 Cell Lysate
Application Notes
SLC38A2 antibody can be used for detection of SLC38A2 by ELISA at 1:1562500. SLC38A2 antibody can be used for detection of SLC38A2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SLC38A2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.