Application
Immunofluorescence of slitrk1 in human brain tissue with slitrk1 antibody at 20 μg/mL.Application
Immunohistochemistry of Slitrk1 in human brain tissue with Slitrk1 antibody at 2.5 μg/mL.Application
Western blot analysis of Slitrk1 in human brain tissue lysate with Slitrk1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | Slitrk1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Slitrk1 Antibody: TTM, LRRC12, KIAA1910, UNQ233/PRO266, SLIT and NTRK-like protein 1, Leucine-rich repeat-containing protein 12 |
| Product description | Slitrk1 Antibody: SLIT and NTRK-like family 1 (Slitrk1) is a member a protein family consisting of six homologous transmembrane proteins (Slitrk1-6) that share two conserved leucine-rich repeat domains in the extracellular domain and have significant homology to Slit, a secreted axonal growth-controlling protein. These proteins are also homologous to trk neurotrophin receptors in their intracellular domains. Expression of Slitrk proteins is highly restricted to neural and brain tumor tissues, but varies within the family. For example, Slitrk1 is expressed primarily in mature neurons. Overexpression of Slitrk1 in transfected neuronal cells induced unipolar neurites, while expression of the other Slitrk proteins inhibited neurite outgrowth, suggesting that these proteins are involved in the control of neurite outgrowth. While Slitrk1 variants have been suggested associated with Tourette's Syndrome, it is thought to play only a minor role if at all.1) Aruga J and Mikoshiba K. Identification and characterization of Slitrk, a novel transmembrane protein family controlling neurite outgrowth. Mol. Cell Neurosci.2003; 24:117-29. |
| Immunogen | Slitrk1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Slitrk1. The immunogen is located within amino acids 630 - 680 of Slitrk1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| Slitrk1 antibody can be used for detection of Slitrk1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLITRK1 |
|---|---|
| Alternative Names | Slitrk1 Antibody: TTM LRRC12 KIAA1910 UNQ233/PRO266 SLIT and NTRK-like protein 1 Leucine-rich repeat-containing protein 12 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | This antibody is predicted to have no cross-reactivity to other Slitrk proteins. |
Application
Immunofluorescence of slitrk1 in human brain tissue with slitrk1 antibody at 20 μg/mL.
Application
Immunohistochemistry of Slitrk1 in human brain tissue with Slitrk1 antibody at 2.5 μg/mL.
Application
Western blot analysis of Slitrk1 in human brain tissue lysate with Slitrk1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
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| Application Notes | Slitrk1 antibody can be used for detection of Slitrk1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Slitrk1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Slitrk1 Antibody is supplied in PBS containing 0.02% sodium azide. |
