Application
Immunofluorescence of Slug in human kidney tissue with Slug antibody at 20 μg/mL.Application
Western blot analysis of Slug in 293 cell lysate with Slug antibody at in (A) 0.5, (B) 1 and (C) 2 μg/mL.| Product Name | Slug Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Slug Antibody: SLUG, WS2D, SLUGH1, SNAIL2, SLUG, SLUGH, Zinc finger protein SNAI2, Neural crest transcription factor Slug |
| Product description | Slug Antibody: Slug, a member of the Snail family of C2H2-type zinc finger transcription factors, was initially identified to be involved in epithelial-mesenchymal transitions as well as the formation of the neural tube during vertebrate embryogenesis. Like Snail, Slug transcription can be induced by growth factors such as FGF, BMP, and TGF-beta. Once expressed, Slug will bind E-box regions of promoters and repress transcription of genes such as E-cadherin and Claudin-. More recently, its expression in breast, esophogeal, and colorectal carcinomas has been correlated with poor prognosis for survival. Furthermore, Slug can protect hemapoietic progenitor cells from radiation-induced apoptosis by repressing the p53-mediated transcription of Puma, a BH3-only antagonist of the anti-apoptotic members of the Bcl-2 family.1) Neito MA, Sargent MG, Wilkinson DG, et al. Control of cell behavior during vertebrate development by Slug, a zinc finger gene. Science 1994; 264:835-9. |
| Immunogen | Slug antibody was raised against a 13 amino acid synthetic peptide from near the center of human Slug. The immunogen is located within amino acids 90 - 140 of Slug. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Slug antibody can be used for detection of Slug by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SNAI2 |
|---|---|
| Alternative Names | Slug Antibody: SLUG WS2D SLUGH1 SNAIL2 SLUG SLUGH Zinc finger protein SNAI2 Neural crest transcription factor Slug |
| Molecular Weight(MW) | Predicted: 29 kDa Observed: 30 kDa |
| Sequence Similarities | 3) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | This Slug antibody is predicted to not cross-react with Snail. |
Application
Immunofluorescence of Slug in human kidney tissue with Slug antibody at 20 μg/mL.
Application
Western blot analysis of Slug in 293 cell lysate with Slug antibody at in (A) 0.5, (B) 1 and (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1210 - HEK293 Cell Lysate |
|---|---|
| Application Notes | Slug antibody can be used for detection of Slug by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Slug antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Slug Antibody is supplied in PBS containing 0.02% sodium azide. |
