Application
Immunofluorescence staining of methanol-fixed HeLa cells using Smad1 (Ab-465).Application
Western blot analysis of lysed extracts from HeLa cells using Smad1 (Ab-465).| Product Name | Smad1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD).1) Hiroaki Seto, et al. (2004) J Clin Invest. March 1; 113 (5): 718–726Linyu Li, et al. (2004) Mol Cell Biol. January; 24 (2): 856–864. Claire Nourry, et al. (2004) BMC Cell Biol. 5: 20 |
| Immunogen | Smad1 (Ab-465) antibody was raised against a peptide sequence around aa. 461~465 (I-S-S-V-S) derived from Human Smad1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFWB |
| Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMAD1 |
|---|---|
| Alternative Names | BSP1 JV41 BSP-1 JV4-1 MADH1 MADR1 Mad-related protein 1 SMAD 1 hSMAD1 Transforming growth factor-beta-signaling protein 1 |
| Molecular Weight(MW) | 55 kDa |
| Entrez Gene | 4086 |
|---|---|
| Protein Accession | NP_001003688.1 |
Application
Immunofluorescence staining of methanol-fixed HeLa cells using Smad1 (Ab-465).
Application
Western blot analysis of lysed extracts from HeLa cells using Smad1 (Ab-465).| Application Notes | Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
