Application
Antibody used in WB on r-SMAD1 at: 1:1000 (Lane 1: 5ug of transfected 293T lysate (SMAD1) , Lane 2: 5ug of transfected 293T lysate (SMAD2) , Lane 3: 5ug of transfected 293T lysate (SMAD3) , Lane 4: 5ug of transfected 293T lysate (SMAD4) , Lane 5: 5ug of transfected 293T lysate (SMAD5) , Lane 6: 5ug of transfected 293T lysate (SMAD6) , Lane 7: 5ug of transfected 293T lysate (SMAD7) , Lane 8: 5ug ofApplication
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.| Product Name | SMAD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SMAD1, BSP1, JV4-1, JV41, MADH1, MADR1, BSP-1 |
| Product description | SMAD1 belongs to the SMAD family. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD1 mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, SMAD1 can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of SMAD1 forms a complex with SMAD4, which is important for its function in the transcription regulation. SMAD1 is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation.The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.1) Blount, A.L., (2008) J. Biol. Chem. 283 (11), 7016-7026. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SMAD1 antibody can be used for detection of SMAD1 by ELISA at 1:12500. SMAD1 antibody can be used for detection of SMAD1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMAD1 |
|---|---|
| Alternative Names | SMAD1 BSP1 JV4-1 JV41 MADH1 MADR1 BSP-1 |
| Molecular Weight(MW) | 52 kDa |
Application
Antibody used in WB on r-SMAD1 at: 1:1000 (Lane 1: 5ug of transfected 293T lysate (SMAD1) , Lane 2: 5ug of transfected 293T lysate (SMAD2) , Lane 3: 5ug of transfected 293T lysate (SMAD3) , Lane 4: 5ug of transfected 293T lysate (SMAD4) , Lane 5: 5ug of transfected 293T lysate (SMAD5) , Lane 6: 5ug of transfected 293T lysate (SMAD6) , Lane 7: 5ug of transfected 293T lysate (SMAD7) , Lane 8: 5ug of
Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | SMAD1 antibody can be used for detection of SMAD1 by ELISA at 1:12500. SMAD1 antibody can be used for detection of SMAD1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SMAD1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
